摘  要：選擇槲皮素、葛根素、黃芩苷、朝藿定C和野黃芩苷為研究對象，在負離子檢測方式下，采用電噴霧-質譜法（ESI-MS）研究A環不同取代基對自身裂解途徑的影響。結果發現，該類化合物主要發生了脫糖、脫CO、脫水和RDA反應，進一步分析發現，A環上不同的取代基對自身質譜裂解途經影響較大，其裂解途徑有顯著差異，且H2O分子的丟失可以成為判斷A環取代基信息的一種方法。A環取代基不同黃酮類化合物的質譜裂解規律有助于復雜黃酮類化合物的快速檢測和結構解析。
關鍵詞：電噴霧-質譜；黃酮類化合物；裂解特征；RDA裂解
中圖分類號：TQ406.7+2；TQ463+.24；O657.63；R927.2    文獻標志碼：A     文章編號：1674-5124（2013）05-0059-03
Study on different substituents in A-ring effect on fragmentation pathway of
flavonoids using electrospray ion trap mass spectrometry
LIN Hong-ying1， HUANG Jian-mei1， DUAN Tian-xuan1， TAN Peng1， LIU Yong-gang1， LI Jiang-ning2
（1. Beijing University of Chinese Medicine，Beijing 100102，China；
2. Beijing Drug Administration，Beijing 100053，China）
Abstract: Under negative ion mode， electrospray ionization mass spectrometry （ESI-MS） was used to study on different substituents in A-ring effect on fragmentation pathway of flavonoids. During the fragmentation， RDA cleavage was detected and CO and H2O were usually lost during the fragmentation. Different substituents in A-ring affected fragmentation obviously， and among them， the loss of H2O molecular could be a marker to judge the substituent in A-ring. The results will be helpful in identifying the structures of flavonoids rapidly with the method of mass spectrometry.
Key words: ESI-MS； flavonoids； fragmentation； RDA cleavage