崔  瑩 （華東師范大學河口海岸學國家重點實驗室，上海 200062）   摘  要: 天然磷脂是含磷酸的類脂化合物，廣泛存在于自然界中，在工業生產、食品科學、醫藥學、生命科學等研究方面都有重要的作用。磷脂的研究手段多樣，高效液相色譜在磷脂的分離、檢測方面具有優勢。結合近年來國內外對高效液相色譜分離磷脂的研究報道，分析了液相色譜分離磷脂所用固定相、流動相、檢測器等關鍵因素，認為液相色譜與質譜聯用是未來液相色譜分析磷脂的技術方向。   關鍵詞：天然磷脂；高效液相色譜法；分離；檢測   中圖分類號：TQ645.9+7      文獻標識碼：A     文章編號：1672-4984（2007）01-0060-02   Advance of phospholipids analysis by high performance liquid chromatography CUI Ying （State Key Laboratory of Estuarine and Coastal Research，East China Normal University，Shanghai 200062，China）   Abstract: Phospholipids are mixtures of lipids containing phosphorus. They widely distribute in the nature. They have great application in the industry，food science，medical science and life science. There are several methods to separate and detect the phospholipids，one of these is the high performance liquid chromatography. This paper analyzed the system used to separate and detect phospholipids and pointed out the combination of the HPLC with MS is the most promising method for separation and detection of phospholipids in the future.   Key words: Natural phospholipids；High performance liquid chromatography；Separation；Detection